Otwinowski Lab

- People - Dominika Borek

Postdoctoral Researcher - CV

Department of Biochemistry
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd., ND10.214
Dallas, Texas 75390-9038

Phone: +1 214-645-6378
Fax: +1 214-645-6353
E-mail:

Education:
Postdoctoral trainee - Biochemistry, University of Texas Southwestern Medical Center, July 2001- August 2005
Ph.D. - Physical Chemistry, Adam Mickiewicz University, Poznan, Poland, September 1997- June 2001
M.Sc. - Chemistry, Adam Mickiewicz University, Poznan, Poland, October 1992 – June 1997

Honors and Awards:

  • NARSAD (National Alliance for Research On Schizophrenia and Depression) Young Investigator Award
  • Adam Mickiewicz University Rector Award for Outstanding Ph.D. Thesis
  • Polish Chemical Society Award for Outstanding Graduate Research
  • Faculty of Chemistry Dean Award for Outstanding M.Sc. Thesis

Research Interest:

  • Methods in macromolecular crystallography
  • Electron microscopy (tomography, single particle reconstruction)
  • Radiation induced changes in diffraction data
  • Difficult structures

Bibliography:

  • Michalska, K., Borek, D., Hernandez-Santoyo, A. & Jaskolski, M. (2008) Crystal packing of plant-type L-asparaginase from Escherichia coli. Acta Cryst. D64: 309-320 Link
  • Rezacova, P., Borek, D., Moy, S.F., Joachimiak A. & Otwinowski Z. (2008) Crystal structure and putative function of small Toprim domain containing protein from Bacillus stearothermophilus. Proteins: Structure, Function and Bioinformatics 70: 311-319. Link
  • Rezacova, P., Krejcirikova, V., Borek, D., Moy, S.F., Joachimiak A. & Otwinowski Z. (2007) The crystal structure of the effector-binding domain of the trehalose repressor TreR from Bacillus subtilis reveals a unique quarternary assembly.
    Proteins: Structure,
    Function and Bioinformatics
    69: 679-682. Link
  • Borek, D., Ginell, S.L., Cymborowski, M., Minor, W. & Otwinowski, Z. (2007) The many faces of radiation damage.
    Journal of Synchrotron Radiation 14: 24-33. pdf
  • Yang, M.J., Gocke, C.B., Luo, X.L., Borek, D., Tomchick, D.R., Machius, M., Otwinowski, Z. & Yu, H.T. (2006)
    Structural basis for CoREST-dependent demethylation of nucleosomes by the human LSD1 histone demetylase. Molecular Cell 23: 377-387. Link
  • Chang, C.I., Chelliah, Y., Borek, D., Mengin-Lecreulx, D. & Deisenhofer, J. (2006) Structure of tracheal cytotoxin in complex
    with a heterodimeric pattern-recognition receptor. Science 311: 1761-1764. Link
  • Borek, D., Michalska, K., Brzezinski, K., Kisiel, A., Podkowinski, J., Bonthron, D.T., Krowarsch, D., Otlewski, J. & Jaskolski, M. (2004) Expression, purification, and catalytic activity of Lupinus luteus asparagine beta-amidohydrolase and its Escherichia coli homolog.
    Eur. J. Biochem
    ., 271: 3215-3226. Link
  • Borek, D., Minor, W. & Otwinowski, Z. (2003) Measurement errors and their consequences in protein crystallography.
    Acta Cryst.
    D59: 2031-2038. pdf
  • Otwinowski, Z., Borek, D., Majewski, W. & Minor, W. (2003) Multiparametric scaling of diffraction intensities.
    Acta Cryst. A59: 228-234. pdf
  • Kozak, M., Borek, D., Janowski, R. & Jaskolski, M. (2002) Crystallization and preliminary crystallographic studies of five crystal forms of Escherichia coli L-asparaginase II (Asp90Glu mutant). Acta Cryst. D58: 130-132. pdf
  • Borek, D. & Jaskolski, M. (2001) Sequence analysis of enzymes with asparaginase activity.
    Acta Bioch. Pol. 48: 893-902. pdf
  • Kozak, M., Hayward, B., Borek, D., Bonthron, D.T. & Jaskolski, M. (2001) Expression, purification and preliminary crystallographic studies of human ketohexokinase. Acta Cryst. D57: 586-588. pdf
  • Borek, D. & Jaskolski, M. (2000) Crystallization and preliminary crystallographic studies of a new L-asparaginase encoded by the Escherichia coli genome. Acta Cryst. D56: 1505-1507. pdf

Other publications:

  • Borek, D., Podkowinski, J., Kisiel, A. & Jaskólski, M. (1999) Isolation and Characterization of cDNA Encoding L-asparaginase from Lupinus luteus (Accession No. AF112444). (PGR99-050) Plant Physiol. 119: 156

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